Product Info Summary
SKU: | P01388 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody
SKU/Catalog Number
P01388
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody catalog # P01388. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01388)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EGA-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-TrkB (Y817)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01388 is reactive to NTRK2 in Human, Mouse, Rat
Applications
P01388 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
130 kDa
Calculated molecular weight
91.999kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:30
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TrkB using anti-TrkB antibody (P01388).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkB antigen affinity purified monoclonal antibody (Catalog # P01388) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkB at approximately 130 kDa. The expected band size for TrkB is at 92 kDa.
Click image to see more details
Western blot analysis of Phospho-TrkB (Y817) expression in SH-SY5Y cell lysate treated with BDNF.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded mouse brain cancer, using Phospho-TrkB (Y817) Antibody.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For NTRK2 (Source: Uniprot.org, NCBI)
Gene Name
NTRK2
Full Name
BDNF/NT-3 growth factors receptor
Weight
91.999kDa
Superfamily
protein kinase superfamily
Alternative Names
BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; NTRK2; TrkB tyrosine kinase; TrkB; trk-B; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B NTRK2 DEE58, EIEE58, GP145-TrkB, OBHD, TRKB, trk-B neurotrophic receptor tyrosine kinase 2 BDNF/NT-3 growth factors receptor|BDNF-tropomyosine receptor kinase B|neurotrophic tyrosine kinase receptor type 2|tropomyosin-related kinase B|tyrosine kinase receptor B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NTRK2, check out the NTRK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NTRK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody (P01388)
Hello CJ!
No publications found for P01388
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Phospho-TrkB (Y817) NTRK2 Rabbit Monoclonal Antibody
Question
My lab would like using your anti-Phospho-TrkB (Y817) Rabbit Monoclonal antibody for modulating synaptic transmission studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
I appreciate your inquiry. This P01388 anti-Phospho-TrkB (Y817) Rabbit Monoclonal antibody is tested on mouse brain. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-28
Question
We have been able to see staining in mouse amygdala. Any tips? Is anti-Phospho-TrkB (Y817) Rabbit Monoclonal antibody supposed to stain amygdala positively?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
Based on literature amygdala does express NTRK2. Based on Uniprot.org, NTRK2 is expressed in hypothalamus, hippocampus, brain, amygdala, plasma, among other tissues. Regarding which tissues have NTRK2 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 7823156, 15164053, 15489334
Hippocampus, Pubmed ID: 7789988, 7936202
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-04-15
Question
My team were well pleased with the WB result of your anti-Phospho-TrkB (Y817) Rabbit Monoclonal antibody. However we have been able to see positive staining in amygdala cell membrane using this antibody. Is that expected? Could you tell me where is NTRK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
According to literature, amygdala does express NTRK2. Generally NTRK2 expresses in cell membrane. Regarding which tissues have NTRK2 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 7823156, 15164053, 15489334
Hippocampus, Pubmed ID: 7789988, 7936202
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2018-07-06
Question
Our lab used your anti-Phospho-TrkB (Y817) Rabbit Monoclonal antibody for IF on amygdala a few years ago. I am using human, and We are going to use the antibody for ICC next. Our lab want to know about examining amygdala as well as hypothalamus in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
A. Jones
Verified customer
Asked: 2015-09-11
Answer
I viewed the website and datasheets of our anti-Phospho-TrkB (Y817) Rabbit Monoclonal antibody and I see that P01388 has been tested on human in both IF and ICC. Thus P01388 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-09-11
Question
We are currently using anti-Phospho-TrkB (Y817) Rabbit Monoclonal antibody P01388 for rat tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
N. Rodriguez
Verified customer
Asked: 2014-10-27
Answer
The anti-Phospho-TrkB (Y817) Rabbit Monoclonal antibody (P01388) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-27